Genotyping of HF Crossbred Breeding Bulls for A1/A2 Variant of β-Casein Gene

Background: The variants of β-casein (CSN 2) gene viz. A1 and A2 are supposed to be related with human health. Hence, know the status breeding bulls used for AI services were screened variants. Total 47 blood samples crossbred from Frozen Semen Laboratory, Pune Aurangabad present work. Methods: extraction genomic DNA by phenol-chloroform method carried out bulls. Further, based PCR-RFLP was identify A1, Pure fragments b-casein amplified PCR using suitable pairs primer amplify 121 bp 244 products. allele specific. fragment then subjected restriction enzyme digestion. digestions resulted into two types band pattern. validation bp. Result: Two genotypes i.e. A1A2 A2A2 revealed in experimental population HF genotypic frequency estimated 65 35 per cent, respectively. may suggested planning future strategy dairy cattle.